Introductory Laboratory

Into. to Microbes and the Immune System Laboratory (MIC 304) Intro. to Microbes and the Immune System Laboratory (MIC 304)
Introductory Microbes and the Immune System Laboratory (MIC 304)

Exercise #

Techniques

0

Lab Regulations

Microscope 101

1

Bunsen Burner Use

Stabs vs. Loops

Aseptic Removal

Aseptic Inoculation

Types of Media

4-Quadrant Streaking

2

Motility Detection

Hanging Drop

Wet Mount

Semi-solid Agar

3

Smear Preparation

Simple stain

Gram Stain

4

Morphological Features

Negative Staining

Acid-fast staining (Ziehl-Neelsen Method)

Spore Stain (Schaeffer-Fulton Method)

5

(days 1 & 2)

Carbohydrate Utilization

Starch Hydrolysis

Casein Hydrolysis

Gelatin Hydrolysis

Blood Agar 

5

(days 3 & 4)

MR/VP Procedure

Indole Test

Citrate Test

Hydrogen Sulfide Production

Catalase Procedure 

6

Differential vs. Selective Media

Mannitol-Salt Agar

Tellurite- Glycine Agar

Brain-Heart Infusion Agar

7 Parasitology Slides 
8

Indicator organisms 

Membrane Filter Technique

Quantitative Examination/Dilutions

Most Probable Number (MPN) Procedure

9

 Antibiotics and Antibiotics

Filter-Paper Disk Method

Kirby-Bauer Method

10

Unknown Project 

2 unknown organisms mixed in one tube

separate and identify

11

 Antibodies

ELISA

12

Slide agglutination

Blood typing

Test your skills 

13

 Bacterial Transformation

Forensic PCR

TESTING (Lowest score of Exam 1, 2 or Practical can be dropped)

Each 25%

Exam 1

Exam 2

Unknown Report 

Practical Exam

Quizzes

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